在单细胞水平检查微生物的异质群体使用稳定化的乳液

日期
周四,2019年11月7日 - 下午02:00 - 周四,2019年11月7日 - 03:00 PM
活动地址
斯坦曼厅,275修道院大街,NY 10031
活动地点
ST-254
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研讨会

 

机械工程系

在单细胞水平检查微生物的异质群体使用稳定化的乳液

教授。康尼湾昌,化学和生物工程,蒙大拿州立大学的系,生物膜工程中心

 

抽象

Conventional methods in microbiology can be limited by long assay execution and analysis times, large reagent volumes, and high single-use supply costs. These limitations can be overcome using drop-based microfluidics in which picoliter-sized, water-in-oil emulsions serve as independent microreactors, allowing for the compartmentalization of microbes and high-throughput assaying at the single cell level. Here, drop-based microfluidics is used to interrogate the physiological heterogeneity of P. aeruginosa cells in a microbial population using a technique we name DropSOAC (Drop Stabilization On A Chip). The DropSOAC method stabilizes the position and volume of monodisperse water-in-oil drops with diameters <20 mm within a monolayer array on a microfluidic chip for 24 h. The stability of drops is maintained by soaking the device in a reservoir containing both water and oil in thermodynamic equilibrium. This ensures that phase equilibrium of the drop emulsion fluids within the porous PDMS material structure is maintained during drop incubation and imaging. Continuing this work, we aim to study the rapid emergence of antibiotic resistance now observed in common bacterial strains to find effective treatments for persistent bacterial infections. This will be performed using a microfluidic chip that is capable of encapsulating in parallel, 96 barcoded assay samples in drops using fluorescent particles. Using a custom-built microscope that can read fluorescence from drops at rates of thousands per second, we demonstrate sorting for a particular barcode combination from our droplet library. The results presented here show the potential of drop-based microfluidics for high-throughput assaying of heterogeneous populations of microbes at the single cell level.

博士。康尼湾钱伟长是在化学和生物工程中心在蒙大拿州立大学的部门生物膜工程的助理教授。博士。钱伟长是韦尔斯利学院毕业,并获得了博士学位从加州大学洛杉矶分校。博士。昌是在物理哈佛大学博士后学者及工程和应用科学学院。博士。昌的研究领域包括软物质,复杂流体,生物材料和微流体。她目前正在研究如何流感病毒的亚群还不如在细菌生物膜社区的新型个体微生物的治疗以及如何空间异质性的应用影响的自发行为,如抗生素耐药性。

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